Hydrogen energy is a kind of secondary energy with an abundant source, wide application, green, and is low-carbon, which is important for building a clean, low-carbon, safe, and efficient energy system and achieving the goal of carbon peaking and being carbon neutral. In this paper, the effect of nozzle position, hydrogen injection timing, and ignition timing on the in-cylinder combustion characteristics is investigated separately with the 13E hydrogen engine as the simulation object. The test results show that when the nozzle position is set in the middle of the intake and exhaust tracts (L2 and L3), the peak in-cylinder pressure is slightly higher than that of L1, but when the nozzle position is L2, the cylinder pressure curve is the smoothest, the peak exothermic rate is the lowest, and the peak cylinder temperature is the lowest. When the ignition timing is consistent, with the delay of hydrogen injection timing, the peak in-cylinder pressure decreases and the peak phase remains