Gasoline direct-injection (GDI) engines have evolved as a solution to meet the current demands of the automotive industry. Benefits of a GDI engine include good fuel economy, good transient response, and low cold start emissions. However, they suffer from problems, like combustion instability, misfire, and impingement of fuel on in-cylinder surfaces. Therefore, to highlight the influence of fuel injection timing on in-cylinder flow, turbulence, mixture distribution and wall impingement, a computational study is conducted on a small-bore GDI engine. Results showed that air motion inside the engine cylinder is influenced by direct-injection of fuel, with considerable variation in turbulent kinetic energy at the time of injection. Due to charge cooling effect, mixture density and trapped mass were increased by about 10.8% and 9.5%, respectively. A significant drop in mean in-cylinder temperature (about 100 °C) was observed with direct-injection of fuel as compared to the case without