Particulate Contamination in Biodiesel Fuel under Long-Term Storage

Many incidents associated with filter plugging have extensively been reported in microbially contaminated diesel and biodiesel fuel systems, especially under long term storage conditions. In this study a quantitative assessment of the undesirable insoluble solids produced in contaminated biodiesel fuels was carried out in order to evaluate their evolution rate during biodeterioration. For this purpose, a series of contaminated biodiesel fuel microcosms were prepared and stored for six months under stable conditions. The quantity of the particulate contaminants was monitored during storage by a multiple filtration technique which was followed at the end by a comparison with the active bioburden per ATP bioluminescence protocol. Additionally, identical microcosms were treated with a commercially available biocide in order to examine the latter’s activity both on solids formation and the microbial proliferation.